HPLC Program: [Insert Analyte Name/Matrix]

Instrument: [e.g., Agilent 1260 Infinity II, Waters Alliance e2695] Column: [e.g., C18, 150 x 4.6 mm, 5 µm] Detection: [e.g., UV-Vis at 254 nm / PDA / Fluorescence]

Detector Program (DAD)

Channel 1: 243 nm (paracetamol max)

Channel 2: 254 nm (parabens)

Reference: 360 nm (baseline correction)

Instructions:

Preparation: Ensure the HPLC system is properly maintained, and the column is equilibrated.

Sample Preparation: Prepare samples according to the protocol (e.g., dissolution, centrifugation).

System Setup: Set the column temperature to 30°C.

Mobile Phase Setup: Prepare the mobile phases, degas them, and load them into the HPLC system.

Injection: Inject the sample (10 μL) into the system.

Run: Start the HPLC program.

Data Analysis: Collect and analyze data from the UV and MS detectors.

Program Fixed — Hplc

HPLC Program: [Insert Analyte Name/Matrix]

Instrument: [e.g., Agilent 1260 Infinity II, Waters Alliance e2695] Column: [e.g., C18, 150 x 4.6 mm, 5 µm] Detection: [e.g., UV-Vis at 254 nm / PDA / Fluorescence]

Detector Program (DAD)

Channel 1: 243 nm (paracetamol max)
Channel 2: 254 nm (parabens)
Reference: 360 nm (baseline correction)

Instructions:

Preparation: Ensure the HPLC system is properly maintained, and the column is equilibrated.
Sample Preparation: Prepare samples according to the protocol (e.g., dissolution, centrifugation).
System Setup: Set the column temperature to 30°C.
Mobile Phase Setup: Prepare the mobile phases, degas them, and load them into the HPLC system.
Injection: Inject the sample (10 μL) into the system.
Run: Start the HPLC program.
Data Analysis: Collect and analyze data from the UV and MS detectors.

1. Mobile Phase Composition

Mobile Phase A: [e.g., Water + 0.1% Formic Acid]
Mobile Phase B: [e.g., Acetonitrile + 0.1% Formic Acid]
Flow Rate: 1.0 mL/min
Injection Volume: 10 µL
Column Temperature: 30 °C
Run Time: 25 minutes